Submit your project details when you are ready to commit resources to the project. If you’d like to discuss your project beforehand, please reach out to firstname.lastname@example.org.
Get started on your gene editing project by emailing us (email@example.com; firstname.lastname@example.org). Whether you are an experienced user of genome editing strategies or you simply have a need to better address your experimental questions, we are happy to provide ideas on strategies and best-practices.
Our staff has extensive experience genome-editing projects. Any one of many design pitfalls can seriously imperil a genome editing project, and we are happy to help guide you through this critical stage. We have designed numerous mouse, rat, Drosophila, and cell-line genome editing projects.
We order reagents (gRNA, expression vectors, donor constructs, single-stranded donor molecules) from highly reputable commercial vendors. Once in-house, we generate purified, injection-grade reagents for rodent embryo microinjection.
Microinjection of embryos to produce gene-edits in mice and rats
Once the necessary reagents are ready, the Animal Models Core will schedule an embryo manipulation day. The gRNA, Cas9, and donor (if applicable) will be microinjected or electroporated into fertilized embryos. The manipulated one-cell embryos are then implanted into the pseudopregnant recipients and offspring are born 19 days (mice) or 22 days (rats) later. At weaning, animals are genotyped to detect presence of edits. Founders can be bred within the Biotechnology Center or shipped to the investigators for breeding.
We provide a number of genotyping solutions most appropriate for your model of choice. We offer high-throughput cell line screening for CRISPR/Cas9 generated knock-out projects. We also regularly genotype F1 litters for mouse and rat models. Please also see our FAQ for genotyping limitations.